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Circulating Tumor Cells

Single Cell Analysis

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Documentation

 

·       Filtration system

·       Single Cell Isolation system

 

 

Videos

 

·       Whole blood filtration + Cell labelling using the CellStainer

·       Isolation of Single Cells

 

 

Publications

 

·       Sanne de Wit, Guus van Dalum, Aufried TM. Lenferink, Arjan GJ Tibbe, T. Jeroen N. Hiltermann, Harry JM Groen, Cees JM van Rijn, Leon WMM Terstappen. The detection of EpCAM+ and EpCAM– circulating tumor cells.  Sci. Rep. 2015;5:12270

·       Joost F Swennenhuis, Arjan GJ Tibbe, Michiel Stevens, Madhu R Katika, Joost van Dalum, Hien Duy Tong, Cees JM van Rijn, Leon WWM Terstappen. Self-seeding microwell chip for the isolation and characterization of single cells. Lab Chip. 2015 Jun 30;15(14):3039-46

·       Sanne de Wit, Guus van Dalum, Joost van Dalum, Aufried Lenferink, Arjan Tibbe, Cees van Rijn, Jeroen Hiltermann, Harry Groen,  Leon Terstappen. Circulating tumor cells in metastatic lung cancer enriched by EpCAM expression and physical characteristics. Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA

·       Joost F. Swennenhuis,  Arjan G.J. Tibbe, Michiel Stevens, Hien Duy Tong, Cees J.M. van Rijn, Leon W.M.M. Terstappen (2014). Single cell isolation and DNA analysis from circulating tumor cells using a self sorting nanowell plate. Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA.

·       Guus van Dalum, Aufried T.M. Lenferink, , Leon W.M.M. Terstappen.  Detection of EpCAM negative circulating tumor cells in CellSearch waste. Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC.

 

 

Posters

 

·       NanoCity 2014

Capture, Detection and Analysis of Circulating Tumor Cells

·       AACR 2014

Circulating tumor cells in metastatic lung cancer enriched by EpCAM expression and physical characteristics.

·       AACR 2014

Single cell isolation and DNA analysis from circulating tumor cells using a self sorting nanowell plate.

·       AACR 2013

Detection of EpCAM negative circulating tumor cells in CellSearch waste

·       XXII Porto Cancer Meeting, 2013

CTCTrap: the European route to a liquid biopsy for all cancer patients

 

 

Books

 

·        Cees JM van Rijn, 2004, Nano and Micro Engineered Membrane Technology, 1st Edition Elsevier Science.